Why headspace gc

The volatile components diffuse into the gas phase until a state of equilibrium is reached. This process can be facilitated through the heating and agitation of the vial.

The sample is then taken from the headspace and can be analyzed. Headspace sampling, therefore, provides a solvent-free extraction of volatile compounds from liquid and solid samples, while eliminating the time-consuming and error-producing steps required in other GC sample-preparation techniques, such as purge and trap or solvent extraction.

The productivity advantages include fewer problems related to carryover, cross-contamination, and foam formation which are typical with purge-and-trap techniques. So, you can extract more data from your samples in less time and at the lowest possible cost per sample.

Samples can be sealed in headspace vials at the point of collection, reducing analyte losses that can occur with other techniques during sample handling and storage. Autosamplers and Sample Prep Workstations. Gas Chromatography. Headspace collection concentrates target analytes, thus improving detection limits a thousandfold compared with classical GC injections.

It also saves time by eliminating the need for labor-intensive solvent. A PerkinElmer application note on headspace analysis of beer illustrates the point. The tens of thousands of commercial, microbrew, and homemade beers consumed worldwide have—as aficionados will tell you—their own unique taste, which producers recognize as the sum contribution of dozens of low-concentration attributes and defects.

But when these compounds exceed certain concentrations, they become undesirable. Acetaldehyde is a by-product of spoilage that drinkers can taste; typical concentrations range up to 20 parts per million.

Trihalomethanes are potentially harmful by-products of municipal water disinfection. Finally, sulfur compounds primarily dimethyl sulfide, sulfur dioxide, and hydrogen sulfide are natural fermentation products that strongly influence taste. Hollender and E.

Hammersley, Chromatography Today 30 Liu and Y. Jiang, Chromatogr. A , Kott and H. Chen, Pharmaceutical Technology 5 , 76 Address: Rupert-Mayer-Str. Product Suggestions. Price on request. Home Basic Principles of Headspace Analysis.

Product was successfully added to your shopping cart. Headspace Vial and Septa Issues In order to achieve the best performance with headspace GC, careful attention should be paid to sample preparation and instrument setup.

It is important to ensure the correct vial and cap are used! Vial Size : Headspace vials are available in 6, 10 and 20 ml sizes. Use a vial large enough to ensure adequate headspace or phase ratio without excessive dilution of the components of interest.

Bottom Profile : Headspace vials may be round-bottomed or flat-bottomed.